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QIAGEN – Sample & Assay Technologies White Papers

QIAGEN – Sample & Assay Technologies

Identifying Meat Species Using RFLP-PCR and the QIAxcel® Advanced System In order to protect consumers’ interests it was necessary to introduce effective control and verify species composition of different food products. Molecular biology PCR-RFPL based method allows for unequivocal species identification in raw but also in processed food. By applying four restriction enzymes it was possible to distinguish meats from 15 animals, and in addition 6 animals served as contamination markers. The automated procedure presented, with the QIAxcel Advanced system, is fast (results available in less than 8h), accurate and therefore applicable in routine analyses.
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Advantages of the QIAxcel System for Bacterial Genotyping Comparative Genomic Fingerprinting (CGF) is an advanced method for C. jejuni subtyping developed at Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, CA. This method generates data that, while highly concordant with MLST, provides additional discriminatory power required for a range of epidemiologic applications, including case cluster detection and outbreak detection. Integration of the QIAxcel Advanced platform into the CGF workflow has led to a high-throughput and low cost subtyping assay that is suitable for deployment in the context of a national laboratory surveillance system.
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High-throughput Detection of Shiga Toxin-Producing Escherichia Coli Using Multiplex PCR and the QIAxcel® System Complex 11multiplex PCR reaction can identify the seven major E. coli serogroups and the four main virulence factors in one PCR reaction. Separating multiplex PCR products with the QIAxcel CE system allows detailed, reliable and fast analyses of a complex samples containing many amplicons within a wide size range. Due to its fast turn-around time and its ability for automatic data interpretation, QIAxcel is an ideal machine for post-PCR separation, visualization and data presentation, especially for multiplex PCR runs in a high-throughput setting.
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Identifying Allergenic Nut Species Using the QIAxcel System This study demonstrated the applicability of the QIAxcel Advanced System for analyzing PCR products as part of an allergen detection workflow. It provided rapid, reliable, and inexpensive identification of four nut species (almond, hazelnut, macadamia nut and peanut).
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